Optimising DNA isolation for medicinal plants
نویسندگان
چکیده
منابع مشابه
Simple and rapid protocol for the isolation of PCR-amplifiable DNA from medicinal plants.
Medicinal plant species has a valuable economic importance because of its usage as pharmaceuticals, nutritional, as well as its use in popular medication. For DNA-based techniques, nanogram quantities of the purified DNA are requisite to amplify and yield sufficient amounts of PCR products. SDS-based DNA isolation method was used to extract DNA from 11 species of different aromatic and me...
متن کاملAssesment of different DNA extraction methods in medicinal plants
Medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. Essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. These compounds especially antioxidants can increase oxidation of DNA in response to wounds. Nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationsh...
متن کاملAssesment of different DNA extraction methods in medicinal plants
Medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. Essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. These compounds especially antioxidants can increase oxidation of DNA in response to wounds. Nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationsh...
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The antiproliferative bioassay-guided fractionation of five Peruvian plants, Doliocarpus dentatus, Picramnia sellowii, Strychnos mitscherlichii, Iryanthera juruensis, and Croton alnifolius, led to the isolation and identification of their different major cytotoxic constituents, betulinic acid (1), nataloe-emodin (2), bisnordihydrotoxyferine (4), 2',4'-dihydroxy-6'-methoxy-3,4-methylenedioxydihy...
متن کاملBioactivity-guided isolation of cytotoxic constituents from three medicinal plants.
CONTEXT The ethanol extracts and their fractions of three Indian medicinal plants, Ervatamia coronaria (Jacq.) Stapf, (Apocynaceae), Mimosa pudica L. (Mimosaceae) and Caesalpinia bonduc (L.) Roxb. (Caesalpiniaceae) were tested for their cytotoxic activity in the brine shrimp lethality (BSL) bioassay and in various cancer cell lines. The plants were selected based on their traditional use in the...
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ژورنال
عنوان ژورنال: South African Journal of Botany
سال: 2008
ISSN: 0254-6299
DOI: 10.1016/j.sajb.2008.07.001